Difference between revisions of "Part:BBa K1998008:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Genes were codon optimised for bacteria using optimiser tool
+
Genes were codon optimised for bacteria.
−
 
+
Lac promoter at the start of the operons.
−
 
+
RBS are present before the start of each gene.
 +
Double terminator present at the end of the operon.
  
 
===Source===
 
===Source===

Latest revision as of 02:06, 18 October 2016


psbELJ-TB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 67
    Illegal AgeI site found at 173
    Illegal AgeI site found at 457
    Illegal AgeI site found at 466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes were codon optimised for bacteria. Lac promoter at the start of the operons. RBS are present before the start of each gene. Double terminator present at the end of the operon.

Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References