Difference between revisions of "Part:BBa K2066104:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K2066104 short</partinfo>
  
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<partinfo>BBa_K2066104 SequenceAndFeatures</partinfo>
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===Design Notes===
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This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”).
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===Source===
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This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.

Latest revision as of 01:46, 18 October 2016

Promoter characterization part - J23151 without RiboJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 106


Design Notes

This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”).


Source

This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.