Difference between revisions of "Part:BBa K2066111:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be completed
+
UNS 6.1 was used as a spacer. This part was created to reduce metabolic strain from maintaining two separate plasmids for the reporter and repressor.
  
  
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===Source===
 
===Source===
  
−
To be completed
+
sfGFP and RBS from Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context” UNS sequences from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”
  
 
===References===
 
===References===

Revision as of 01:22, 18 October 2016


pLacO1 sfGFP + LacI (weak)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1087
    Illegal NheI site found at 1110
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 224


Design Notes

UNS 6.1 was used as a spacer. This part was created to reduce metabolic strain from maintaining two separate plasmids for the reporter and repressor.


Source

sfGFP and RBS from Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context” UNS sequences from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”

References