Difference between revisions of "Part:BBa K1998003:Design"
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===Design Notes=== | ===Design Notes=== | ||
Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact. | Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact. | ||
| − | + | Lac promoter at the start of the operon. | |
| − | + | RBS are present before the start of the gene. | |
| + | Double terminator present at the end of the operon. | ||
===Source=== | ===Source=== | ||
Latest revision as of 00:06, 18 October 2016
psbD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact. Lac promoter at the start of the operon. RBS are present before the start of the gene. Double terminator present at the end of the operon.
Source
The genes were sourced from Chlamydomonas rienhardtii and synthesised.