Difference between revisions of "Part:BBa K1899005"
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The fold change between<b> pSB3K3-PhlFp - BBa_E0240(A)</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D)</b> is around 13.2 times and 7.01 times respectively.
 
The fold change between<b> pSB3K3-PhlFp - BBa_E0240(A)</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D)</b> is around 13.2 times and 7.01 times respectively.
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This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(tetR). The toxicity reduces the growth rate of the<i> E. coli</i>  containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of <i> PhlFp</i>  at this stage.
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This is due to the toxicity of <bbpart>BBa_C0040 <bbpart>(tetR). The toxicity reduces the growth rate of the<i> E. coli</i>  containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of <i> PhlFp</i>  at this stage.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 23:32, 17 October 2016


J23101-B0032-TetR-B1006- phlFp -GFP

This part is constructed for investigating the effect of tetR over PhlFp.

Results

748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) and pSB3K3-PhlFp- BBa_E0240(A)

The fold change between pSB3K3-PhlFp - BBa_E0240(A) and negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) is around 13.2 times and 7.01 times respectively.

This is due to the toxicity of BBa_C0040