Difference between revisions of "Part:BBa K1899005"

Revision as of 23:31, 17 October 2016

J23101-B0032-TetR-B1006- phlFp -GFP

This part is constructed for investigating the effect of tetR over PhlFp.

Results

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) and pSB3K3-PhlFp- BBa_E0240(A)

The fold change between pSB3K3-PhlFp - BBa_E0240(A) and negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) is around 13.2 times and 7.01 times respectively.

This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(tetR). The toxicity reduces the growth rate of the E. coli containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of PhlFp at this stage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1539

@@ Line 6: / Line 6: @@
 <br>
 ===Results===
-https://static.igem.org/mediawiki/parts/thumb/2/25/.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg
-<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240 and  pSB3K3-<i>PhlFp</i>- BBa_E0240</b>
+https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg
-The fold change between<b> pSB3K3-PhlFp - BBa_E0240</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240</b> is around 13.2 times and 7.01 times respectively.
+<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) and  pSB3K3-<i>PhlFp</i>- BBa_E0240(A)</b>
-This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(TetR). The toxicity reduces the growth rate of the<i> E. coli</i>  containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of <i> PhlFp</i>  at this stage.
+The fold change between<b> pSB3K3-PhlFp - BBa_E0240(A)</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D)</b> is around 13.2 times and 7.01 times respectively.
+This is due to the toxicity of <bbcode>BBa_C0040 <bbcode>(tetR). The toxicity reduces the growth rate of the<i> E. coli</i>  containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say TetR interferes the functionality of <i> PhlFp</i>  at this stage.
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