Difference between revisions of "Part:BBa K2100001:Experience"

 
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===Applications of BBa_K2100001===
 
===Applications of BBa_K2100001===
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We characterized the synthetic ERE5 promoter in three cell lines: MCF-7, tHESC, and ISH. All cell lines have endogeneous Estrogen Receptor alpha. We analyzed data from cells induced with estradiol (E2) and uninduced as a control.
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Experiment in MCF-7:
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We transfected MCF-7 cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.
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https://static.igem.org/mediawiki/2016/b/b8/T--MIT--pERE5MCF7.png
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Experiment in tHESC:
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We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:08, 17 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K2100001

We characterized the synthetic ERE5 promoter in three cell lines: MCF-7, tHESC, and ISH. All cell lines have endogeneous Estrogen Receptor alpha. We analyzed data from cells induced with estradiol (E2) and uninduced as a control.

Experiment in MCF-7:

We transfected MCF-7 cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

T--MIT--pERE5MCF7.png

Experiment in tHESC:

We transfected tHESC cells with 250ng of hEF1a-mKate as a transfection marker and 250 ng pERE5-eYFP to examine the promoter's transcriptional activity by observing increases in yellow fluorescence upon cells being induced with 5 nM E2.

User Reviews

UNIQa8197e6ac82f1583-partinfo-00000000-QINU UNIQa8197e6ac82f1583-partinfo-00000001-QINU