Difference between revisions of "Part:BBa K2136002"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2136002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2136002 SequenceAndFeatures</partinfo>
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PCR from pSB1C3 + Lysostaphin
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https://static.igem.org/mediawiki/2016/9/93/T--USP_UNIFESP-Brazil--Lysosfromplasmid.png">
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<div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/9/93/T--USP_UNIFESP-Brazil--Lysosfromplasmid.png')">
  
  

Revision as of 22:39, 17 October 2016


Lysostaphin

Lysostaphin is a zinc metalloproteinase (EC 3.4.24.75) produced by Staphylococcus simulans. This enzyme hydrolyses Glycine/Glycine bonds in the pentaglycine interpeptide that maintain the stability of peptidoglycans in staphylococcal cell wall [1]. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms and due to its specificity, it could have high potential in the treatment of antibiotic-resistant staphylococcal infections [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 505
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

PCR from pSB1C3 + Lysostaphin


T--USP_UNIFESP-Brazil--Lysosfromplasmid.png">