Difference between revisions of "Part:BBa K2008008:Design"

 
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===Design Notes===
 
===Design Notes===
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Addition of the AmyE homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.
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Addition of the ThrC homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.
  
  

Revision as of 20:59, 17 October 2016


pVeg->sec-TD1 modular secretion platform with ThrC homology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of the ThrC homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.


Source

The pVeg promoter, sec secretion tag as well as the 125-bp AmyE regions of homology B. subtilis genome. TD1 is a transdermal tag first entered into the registry by the USTC_China iGEM team in 2013.

References