Difference between revisions of "Part:BBa K1930004:Design"

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===Design Notes===
 
===Design Notes===
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To design a ciprofloxacin resistance cassette we designed a gBlock that contains the <em>Bacillus subtilis</em> promoter P<sub>AtpI</sub>, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original <em>qnrS1</em> gene sequence from <em>E. coli</em>, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts P<sub>AtpI</sub>+RBS+<em>qnrS1</em>+2TER.
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To design a ciprofloxacin resistance cassette we designed a gBlock that contains the <em>Bacillus subtilis</em> promoter P<sub>AtpI</sub>, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original <em>qnrS1</em> gene sequence from <em>E. coli</em>. <em>qnr</em> genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones. Finally, this gBlock contains a double terminator [https://parts.igem.org/Part:BBa_B0015 ''BBa_B0015''] from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts P<sub>AtpI</sub>+RBS+<em>qnrS1</em>+2TER.
  
  

Revision as of 18:37, 17 October 2016


Ciprofloxacin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli. qnr genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones. Finally, this gBlock contains a double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.


Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Gene ID

Quinolone resistance