Difference between revisions of "Part:BBa I712019"
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===Modification===
 
===Modification===
−
In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part[https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006] in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.
+
In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part [https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006] in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.
  
 
For more information, please see our Part page: [https://parts.igem.org/Part:BBa_K1993006 https://parts.igem.org/Part:BBa_K1993006 ]
 
For more information, please see our Part page: [https://parts.igem.org/Part:BBa_K1993006 https://parts.igem.org/Part:BBa_K1993006 ]
Line 17: Line 17:
 
We used the part BBa_I712019 to construct the part with split-luciferase. Our sequencing result suggested that this part is completely working.          --------Tianjin IGEM TEAM
 
We used the part BBa_I712019 to construct the part with split-luciferase. Our sequencing result suggested that this part is completely working.          --------Tianjin IGEM TEAM
  
−
In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part[https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006] in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.
+
In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part [https://parts.igem.org/Part:BBa_K1993006 BBa_K1993006] in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.
  
 
For more information, please see our Part page: [https://parts.igem.org/Part:BBa_K1993006 https://parts.igem.org/Part:BBa_K1993006 ]
 
For more information, please see our Part page: [https://parts.igem.org/Part:BBa_K1993006 https://parts.igem.org/Part:BBa_K1993006 ]

Revision as of 17:58, 17 October 2016

Firefly luciferase - luciferase from Photinus pyralis


Modification

In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part BBa_K1993006 in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.

For more information, please see our Part page: https://parts.igem.org/Part:BBa_K1993006

Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.

Usage and Biology

This part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix. See the sequence first.

We used the part BBa_I712019 to construct the part with split-luciferase. Our sequencing result suggested that this part is completely working. --------Tianjin IGEM TEAM

In iGEM 2016, our team, SYSU-MEDICINE, had constructed a new composite part BBa_K1993006 in order to improve the positioning function of this previous part. Our new composite part contains gene Firefly Luciferase, dTomato and hFTH, which help users observe biological processes in vivo and in vitro.

For more information, please see our Part page: https://parts.igem.org/Part:BBa_K1993006


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808