Difference between revisions of "Part:BBa K1968022:Design"
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Based ont he ilov sequence from BBa_K660004. | Based ont he ilov sequence from BBa_K660004. | ||
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+ | This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing | ||
===References=== | ===References=== |
Latest revision as of 17:03, 17 October 2016
weLOV1 fluorescent protein Phytobrick
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequences can sometimes be troublesome to work with, especially if these are not codon optimized for the selected organism. Since the team was going to work with four different organisms (E. coli, Rhodococcus justt, Penicillium roqueforti, Synechocystis sp. PCC6803) as an alternative to synthesizing the optimized version for each organism a different approach was discussed and implemented.
Designed under the Phytobrick standard.
Source
Based ont he ilov sequence from BBa_K660004.
This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing