Difference between revisions of "Part:BBa K1993033"

Revision as of 16:33, 17 October 2016

CXCR5-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing a chemokine receptor CXCR5 and a bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology. CXCL13 is one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH). CXCR5, its corresponding chemokine receptor, is responsible for homing to the lesions. BBa_K1993013 Details could be seen on. Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. BBa_K1993015 Details could be seen on . T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019 Details could be seen on

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1398
Illegal BamHI site found at 1941
Illegal XhoI site found at 31
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 973
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 259
Illegal SapI site found at 1050

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1993033 short</partinfo>
-CXCR5-T2A-luciferase
+With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing a chemokine receptor CXCR5 and a bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.
+CXCL13 is one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH). CXCR5, its corresponding chemokine receptor, is responsible for homing to the lesions.  [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013 Details could be seen on].
+Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. [https://parts.igem.org/Part:BBa_K1993015 BBa_K1993015 Details could be seen on ].
+T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019 Details could be seen on ]
 <!-- Add more about the biology of this part here