Difference between revisions of "Part:BBa K2150017"
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<partinfo>BBa_K2150017 short</partinfo> | <partinfo>BBa_K2150017 short</partinfo> | ||
| − | T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator. | + | T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator. |
The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected. | The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected. | ||
Revision as of 15:56, 17 October 2016
pT7 TetX-GFP(fusion protein)
T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator. The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 608
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1905