Difference between revisions of "Part:BBa K2150015"

 
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<partinfo>BBa_K2150015 short</partinfo>
 
<partinfo>BBa_K2150015 short</partinfo>
  
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This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR. This part can report the expression of TetX with or without tetracycline.
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This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR, and the repression can be inhibited by tetracycline and its analogs. This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli.  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 15:52, 17 October 2016


pTet TetX-GFP(fusion protein)

This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR, and the repression can be inhibited by tetracycline and its analogs. This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939