Difference between revisions of "Part:BBa K2150017"

Revision as of 15:51, 17 October 2016

pT7 TetX-GFP(fusion protein)

T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator. Although it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 608
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1905

@@ Line 4: / Line 4: @@
 T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator.
-Although it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. The fluorescence intensity
+Although it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected.
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