Difference between revisions of "Part:BBa K2150017"
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T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator.
 
T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator.
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TetX and GFP are combined with a 3*GGGGS linker to form a fusion protein. By testing the fluorescence intensity, we found that the expression level of tetX-GFP is lower than single tetX under same circumstances(same promoter and RBS, same living stage of bacteria). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.
+
Although it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. The fluorescence intensity
  
 
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Revision as of 15:42, 17 October 2016


pT7 TetX-GFP(fusion protein)

T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP) with double terminator. Although it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. The fluorescence intensity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1905