Difference between revisions of "Part:BBa K1985015"

Revision as of 15:26, 17 October 2016

pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM]

This part is an improved version of biobrick (BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed and instead an inducible arabinose promoter, part BBa_K1321333, has been used. The second part of this composite biobrick contains 2 segments: the CsgA signal sequence and Sup35NM.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

Usage and Biology

For more information on biology and usage of Sup35NM with the CsgA signal sequence, see part BBa_K1739002. For information on the promoter see BBa_K1321333.

Validation

The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.

File:Filename.jpeg

Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1985015 short</partinfo>
-This part is an improved version of biobrick (BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed.. It contains 2 segments, the CsgA signal sequence and Sup35NM.
+This part is an improved version of biobrick (BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed and instead an inducible arabinose promoter, part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1321333 BBa_K1321333], has been used. The second part of this composite biobrick contains 2 segments: the CsgA signal sequence and Sup35NM.
 <span class='h3bb'>Sequence and Features</span>
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-For more information on biology and usage see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002]
+For more information on biology and usage of Sup35NM with the CsgA signal sequence, see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002]. For information on the promoter see [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1321333 BBa_K1321333].
 ===Validation===