Difference between revisions of "Part:BBa K1985015"

 
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<partinfo>BBa_K1985015 short</partinfo>
 
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This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 2 segments, the CsgA signal sequence and Sup35NM
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This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 2 segments, the CsgA signal sequence and Sup35NM.
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1985015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1985015 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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For more information on biology and usage see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002]
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===Validation===
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The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.
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[[File:filename.jpeg||400px|thumb|centre|Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.]]
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Revision as of 15:21, 17 October 2016


pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM]

This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 2 segments, the CsgA signal sequence and Sup35NM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Usage and Biology

For more information on biology and usage see part BBa_K1739002

Validation

The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.

File:Filename.jpeg
Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.