Difference between revisions of "Part:BBa K1985012"

Revision as of 15:20, 17 October 2016

[CsgA-SS-Sup35NM]

This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 671

Usage and Biology

For more information on biology and usage see part BBa_K1739002

Validation

The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.

File:Filename.jpeg

Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1985012 short</partinfo>
+This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM.
 <!-- -->
@@ Line 6: / Line 8: @@
 <partinfo>BBa_K1985012 SequenceAndFeatures</partinfo>
-This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in on biobrick: CsgA signal sequence and Sup35NM.
 ===Usage and Biology===
 For more information on biology and usage see part [https://parts.igem.org/Part:BBa_K1739002 BBa_K1739002]
+===Validation===
+The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.
+[[File:filename.jpeg||400px|thumb|centre|Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.]]
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