Difference between revisions of "Part:BBa K1951003"
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The protein production of DesD has been tested by our team in a composite parts we designed : | The protein production of DesD has been tested by our team in a composite parts we designed : | ||
* [https://parts.igem.org/Part:BBa_K1951011 BBa_K1951011 : desABCD cluster] | * [https://parts.igem.org/Part:BBa_K1951011 BBa_K1951011 : desABCD cluster] | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
Latest revision as of 14:27, 17 October 2016
desD, desferrioxamine biosynthesis protein coding sequence
DesD, desferrionxamine biosynthesis protein
General information
Monooxygenase is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the last step in the production of desferrioxamine B in Streptomyces coelicolor.
Design summary
All forbidden restriction sites have been removed.
Codons have been optimized for E.coli to allow a maximal transcription level.
Experiment summary
The protein production of DesD has been tested by our team in a composite parts we designed :
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 350
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1440
Illegal BsaI.rc site found at 535