Difference between revisions of "Part:BBa K1937007"

Revision as of 14:00, 17 October 2016

AF-A : operon of antifungal peptides

(Chassis E. coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis) Length: 1006 bp Background: AF-A is a part encoding two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 petide under the control of the strong constitutive promoter pVeg. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 250
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 438

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1937007 short</partinfo>
-(Chassis E.coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis)
+(Chassis <i>E. coli</i>, carrier plasmid pSB1C3, part destined for use in <i>Bacillus subtilis</i>)
 Length: 1006 bp
 Background:
-AF-A is a part designed to be combined with AF-B to form an operon of 5 antifungal peptides. The interest of using several antifungals is to have a broad spectrum of fungi target, because each antifungal has its own characteristics.The peptides were designed to be expressed and secreted by Bacillus subtilis. This operon was also initially designed to be expressed under the control of the pNagA or pNagP promoter (BBa_K1937003 and BBa_K1937005 respectively). In spite of several cloning strategies, the AF-B construction was not obtained, likely due to the toxicity of one of the peptides for Bacillus subtilis.
+AF-A is a part encoding two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 petide under the control of the strong constitutive promoter pVeg.
 This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)