Difference between revisions of "Part:BBa K2172002:Design"
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===Source=== | ===Source=== | ||
− | The source of the part is the vector pGEX-KG | + | The source of the part is the vector pGEX-KG. |
===References=== | ===References=== |
Latest revision as of 13:39, 17 October 2016
Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 747
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 159
Design Notes
Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.
Source
The source of the part is the vector pGEX-KG.