Difference between revisions of "Part:BBa K2172002"
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<partinfo>BBa_K2172002 short</partinfo> | <partinfo>BBa_K2172002 short</partinfo> | ||
| − | This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a GST label for purification, a thrombin restriction enzyme cleavage site and a GFP detection unit. | + | This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site and a GFP detection unit. It also has a promoter and a terminator. |
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Revision as of 13:39, 17 October 2016
Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-
This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site and a GFP detection unit. It also has a promoter and a terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 747
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 159