Difference between revisions of "Part:BBa K1951003"

 
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<partinfo>BBa_K1951003 short</partinfo>
 
<partinfo>BBa_K1951003 short</partinfo>
  
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This coding sequence codes DesD od Streptomyces coelicolor, which is the last protein involved in the metabolic pathway of Desferrioxamine B. This enzyme is called desferrioxamine biosynthesis protein or DesD and allows to transform N-acetyl N-hydroxycadaverine into Desferrioxamine B by the transformation of 3a nucleoside triphosphate into 3a nucleoside 5'monophosphate-3-diphosphate.
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==DesD, desferrionxamine biosynthesis protein==
  
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===General information===
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===Usage and Biology===
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Monooxygenase is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H.
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<span class='h3bb'>Sequence and Features</span>
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It is also the last step in the production of desferrioxamine B in <i>Streptomyces coelicolor</i>.
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[[File:T--Aix-Marseille--pathwayD.jpeg|400px|left|thumb|DesD in the Desferrionxamine B pathway]]
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=== Design summary ===
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All forbidden restriction sites have been removed.
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Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level.
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=== Experiment summary ===
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The protein production of DesD has been tested by our team in a composite parts we designed :
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* [https://parts.igem.org/Part:BBa_K1951011 BBa_K1951011 : desABCD cluster]
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===Sequence and Features===
 
<partinfo>BBa_K1951003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1951003 SequenceAndFeatures</partinfo>
  

Revision as of 13:20, 17 October 2016


desD, desferrioxamine biosynthesis protein coding sequence

DesD, desferrionxamine biosynthesis protein

General information

Monooxygenase is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the last step in the production of desferrioxamine B in Streptomyces coelicolor.

DesD in the Desferrionxamine B pathway

Design summary

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Experiment summary

The protein production of DesD has been tested by our team in a composite parts we designed :


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 350
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1440
    Illegal BsaI.rc site found at 535