Difference between revisions of "Part:BBa K1951002"

 
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<partinfo>BBa_K1951002 short</partinfo>
 
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desC is the coding sequence of DesC which is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.
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==DesA, lysine decarboxylase coding sequence==
  
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<!-- Add more about the biology of this part here
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===General information===
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===Usage and Biology===
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DesC is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.
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It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine.
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[[File:T--Aix-Marseille--pathwayC.jpeg|400px|left|thumb|DesC in the Desferrionxamine B pathway]]
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=== Design summary ===
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All forbidden restriction sites have been removed.
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Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level.
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=== Experiment summary ===
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The following biobrick functionality has been tested by our team in two different composite parts we designed :
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* [https://parts.igem.org/Part:BBa_K1951011 BBa_K1951011 : desABCD cluster - protein production DesC]
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===Sequence and Features===  
  
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K1951002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1951002 SequenceAndFeatures</partinfo>
  

Revision as of 13:03, 17 October 2016


desC, acyl transferase coding sequence

DesA, lysine decarboxylase coding sequence

General information

DesC is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.

It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine.

DesC in the Desferrionxamine B pathway

Design summary

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Experiment summary

The following biobrick functionality has been tested by our team in two different composite parts we designed :

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]