Difference between revisions of "Part:BBa K1951001"
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===Sequence and Features===
 
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Revision as of 12:51, 17 October 2016

DesA, lysine decarboxylase coding sequence

General information

Monooxygenase is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine.

DesA in the Desferrionxamine B pathway

Lysine descarboxylase (Streptomyces coelicolor) is an enzyme from the lyase family that converts lysine to cadaverine.

Design summary

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Experiment summary

The following biobrick functionality has been tested by our team in two different composite parts we designed :


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 569
    Illegal BglII site found at 794
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 221
    Illegal NgoMIV site found at 469
    Illegal AgeI site found at 974
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943