Difference between revisions of "Part:BBa K1951006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| − | + | <partinfo>BBa_K1951005 SequenceAndFeatures</partinfo> | |
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Revision as of 12:39, 17 October 2016
General information about flagellin (FliC protein)
To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951009
This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio can adsorb metallic nanoparticules as gold or platinum. [2]
Design summary
Starting from the Desulfovibrio vulgaris strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951009. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951009 design