Difference between revisions of "Part:BBa K1951006"
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== Design summary == | == Design summary == | ||
| − | Starting from the ''Desulfovibrio vulgaris'' strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 | + | Starting from the ''Desulfovibrio vulgaris'' strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951009]. |
All forbidden restriction sites have been removed. | All forbidden restriction sites have been removed. | ||
Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level. | ||
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This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951009:Design BBa_K1951009 design] | This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951009:Design BBa_K1951009 design] | ||
| − | + | ==Sequence and Features== | |
| + | <partinfo>BBa_K1951006 SequenceAndFeatures</partinfo> | ||
Revision as of 12:22, 17 October 2016
Contents
General information about flagellin (FliC protein)
To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951009
This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio can adsorb metallic nanoparticules as gold or platinum. [1]
Design summary
Starting from the Desulfovibrio vulgaris strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951009. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.
Proof of functionality
This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951009 design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 400
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 400