Difference between revisions of "Part:BBa K1951006"
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This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from ''Desulfovibrio'' can adsorb metallic nanoparticules as gold or platinum. <ref> https://www.ncbi.nlm.nih.gov/pubmed/25686718 </ref>  
 
This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from ''Desulfovibrio'' can adsorb metallic nanoparticules as gold or platinum. <ref> https://www.ncbi.nlm.nih.gov/pubmed/25686718 </ref>  
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== Design summary ==
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Starting from the ''Desulfovibrio vulgaris'' strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in <i>E. coli</i> chassis [https://parts.igem.org/Part:BBa_K1951008 BBa_K1951008].
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All forbidden restriction sites have been removed.
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Codons have been optimized for <i> E.coli </i> to allow a maximal transcription level.
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== Proof of functionality ==
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This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been <u>validated as functional</u>. For further information, go on the following link : [https://parts.igem.org/Part:BBa_K1951009:Design BBa_K1951009 design]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:20, 17 October 2016

FliC Desulfovibrio coding sequence

General information about flagellin (FliC protein)

To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951009

This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio can adsorb metallic nanoparticules as gold or platinum. [1]

Design summary

Starting from the Desulfovibrio vulgaris strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.

Proof of functionality

This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951009 design






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 400


  1. ↑ https://www.ncbi.nlm.nih.gov/pubmed/25686718