Difference between revisions of "Part:BBa K1899006:Experience"

Revision as of 11:49, 17 October 2016

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Applications of BBa_K1899006

Characterisation Result

[[File:T--Hong_Kong_HKUST--Phlfp_C.jpg|thumb|600px|center| Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240

The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) is 13.2, which is same as that of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240<b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between lacp and Phlfp</ii>(BBa_K1899004

@@ Line 7: / Line 7: @@
 Characterisation Result
+[[File:T--Hong_Kong_HKUST--Phlfp_C.jpg|thumb|600px|center|
+<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and  pSB3K3-PhlFp - BBa_E0240</b>
-https://static.igem.org/mediawiki/parts/thumb/3/3c/T--Hong_Kong_HKUST--Phlfp_C.jpg/693px-T--Hong_Kong_HKUST--Phlfp_C.jpg
+The fold change between <b>pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240)</b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240<b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp</i> and <i>Phlfp</ii>(<bbpart>BBa_K1899004<bbpart>). ]]
-[[File:T--Hong_Kong_HKUST--Phlfp_C.jpg|thumb|600px|center|<b>Fig 1. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
-Fig a)<b> Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and  pSB3K3-PhlFp - BBa_E0240</b>
-The fold change between <b>pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240)<b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240<b>. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp<i> and <i>Phlfp<i>(<bbpart>BBa_K1899004<bbpart>).