Difference between revisions of "Part:BBa K1021003"

Revision as of 11:16, 17 October 2016

bar (bialaphos resistance)

The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.

Usage and Biology

The common fungus transformation system of entomopathogen Metarhizium anisopliae was based on resistance to the herbicide glufosinate ammonium (phosphinothricin), conferred by the bar gene.

Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1 in our chassis organism M.anisopliae.

The transformants carrying the bar gene can grow on the PDA containing glufosinate ammonium.

Figure1. The emerged colonies on glufosinate ammonium agar

Figure2. Transformants grow on selective medium

We did the inhibition test, the data is showed below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 545
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 451
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 The common fungus transformation system of entomopathogen Metarhizium anisopliae was based on resistance to the herbicide glufosinate ammonium (phosphinothricin), conferred by the bar gene.
-Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1.
+Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1 in our chassis organism ''M.anisopliae''.
 * The transformants carrying the bar gene can grow on the PDA containing glufosinate ammonium.