Difference between revisions of "Part:BBa K1951005"

Revision as of 11:07, 17 October 2016

General information about flagellin (FliC protein)

To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951008

Flagellin is the main protein which polymerizes to form the filaments of bacterial flagella and is necessary for bacterial movement by swimming. The possession of a flagellum allow bacteria to swim towards food sources and so gives them a selective advantage. ^[1]

It has been demonstrated that Flagellin has the ability to adsorb precious metals such as Platinum, Gold..

Design summary

Starting from the E.coli flagellin sequence ( http://www.uniprot.org/uniprot/P04949 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951008. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.

Proof of functionality

This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951008 design

Sequence and Features

1. ↑ https://www.ncbi.nlm.nih.gov/gene/949101