Difference between revisions of "Part:BBa K1930002"

Latest revision as of 10:55, 17 October 2016

Encryption key sequence in integration plasmid (BBa_K823023)

The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 6045
Illegal XhoI site found at 3553
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1107
Illegal NgoMIV site found at 3517
Illegal NgoMIV site found at 4656
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4480

Revision as of 20:49, 16 October 2016 (view source) Registry (Talk \| contribs)		Latest revision as of 10:55, 17 October 2016 (view source) Barawaclawik (Talk \| contribs)
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	<partinfo>BBa_K1930002 short</partinfo>		<partinfo>BBa_K1930002 short</partinfo>

−	The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for <em>Bacillus subtilis</em>, which can be used for cloning in <em>E. coli</em> as well~~. An RFP is inserted in BBa_K823023 for more efficient screening after transformation~~.	+	The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for <em>Bacillus subtilis</em>, which can be used for cloning in <em>E. coli</em> as well.