Difference between revisions of "Part:BBa K1930007"
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| + | <p>This part is an integration plasmid ([https://parts.igem.org/Part:BBa_K823023 ''BBa_K823023'']) carrying ciprofloxacin resistance cassette ([https://parts.igem.org/Part:BBa_K1930004 ''BBa_K1930004'']). BBa_K823023 is an available BioBrick from iGEM Munich 2012. It is an integration plasmid for <em>Bacillus subtilis</em>, which can be used for cloning in <em>E. coli</em> as well. Ciprofloxacin resistance cassette is containing a P<sub>AtpI</sub> constitutive promoter, ciprofloxacin resistance gene <em>qnrS1</em> and double terminator.</p> | ||
| + | <p>The promoter P<sub>AtpI</sub> ([https://parts.igem.org/Part:BBa_K1930005 ''BBa_K1930005'']) is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.</p> | ||
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| + | <p>Ciprofloxacin resistance gene <em>qnrS1</em> is found naturally in <em>E. coli</em>, and other Gram-negative strains. <em>qnr</em> genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.</p> | ||
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| + | <p> Double terminator ([https://parts.igem.org/Part:BBa_B0015 ''BBa_B0015'']) is the most commonly used terminator. For more information see here: https://parts.igem.org/Part:BBa_B0015. </p> | ||
Revision as of 10:54, 17 October 2016
This part is an integration plasmid (BBa_K823023) carrying ciprofloxacin resistance cassette (BBa_K1930004). BBa_K823023 is an available BioBrick from iGEM Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. Ciprofloxacin resistance cassette is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator.
The promoter PAtpI (BBa_K1930005) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.
Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.
Double terminator (BBa_B0015) is the most commonly used terminator. For more information see here: https://parts.igem.org/Part:BBa_B0015.