Difference between revisions of "Part:BBa K1930006:Design"
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===References=== | ===References=== | ||
− | <p>[http://2016.igem.org/Team:Groningen/Experiments iGEM Groningen 2016''Decoy experiment'']</p> | + | <p>[http://2016.igem.org/Team:Groningen/Experiments iGEM Groningen 2016 ''Decoy experiment'']</p> |
<p>[http://aem.asm.org/content/79/20/6481.full ''Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging'']</p> | <p>[http://aem.asm.org/content/79/20/6481.full ''Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging'']</p> |
Revision as of 10:47, 17 October 2016
sfGFP(Sp)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
sfGFP(Sp) originally optimized for Streptococcus pneumoniae. It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs).
Source
Plasmid pNW
References
[http://2016.igem.org/Team:Groningen/Experiments iGEM Groningen 2016 Decoy experiment]
[http://aem.asm.org/content/79/20/6481.full Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging]