Difference between revisions of "Part:BBa K1937005:Design"
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===Design Notes===
 
===Design Notes===
 
The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
 
The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
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The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.
+
The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the <i>Bacillus</i> genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.
  
 
[[File:BBa_K1937005-map.png]]
 
[[File:BBa_K1937005-map.png]]
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===Source===
 
===Source===
  
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In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.
+
In <i>Bacillus subtilis</i>, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.
  
 
===References===
 
===References===

Revision as of 10:06, 17 October 2016


pNagP-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 201
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.

BBa K1937005-map.png



Source

In Bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.

References