Difference between revisions of "Part:BBa K1937003"

Revision as of 10:02, 17 October 2016

pNagA-RFP

The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis. In Bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 201
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 761
Illegal AgeI site found at 873
1000
COMPATIBLE WITH RFC[1000]

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 pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in <i>Bacillus subtilis.</i>
 In <i>Bacillus subtilis</i>, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate. This NAG molecule is the major component of chitin, a characteristic molecule of fungi.
-<!-- Add more about the biology of this part here ===Usage and Biology===<!-- --><span class='h3bb'>==Sequence and Features==</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- -->
+<!-- Add more about the biology of this part here ===Usage and Biology===<!-- --><span class='h3bb'>Sequence and Features</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- -->