Difference between revisions of "Part:BBa K1959001"

Revision as of 08:46, 17 October 2016

Modification of phytoene desaturase (PDS/CrtI)

This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. A Pea transit peptide is fused with cds of phytoene desaturase in order to stablize and help phytoene desaturase folding in a correct formation in rice (Oryza sativa) cell. Codon optimized has been made for the expression in rice.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 242
Illegal XhoI site found at 1372
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1582
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1959001 short</partinfo>
-This part is the coding sequence of phytoene desaturase from Erwinia uredovora. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. A Pea transit peptide is fused with cds of phytoene desaturase in order to stablize and help phytoene desaturase folding in a correct formation in Oryza sativa cell. Codon optimized has been made for the expression in Oryza sativa.
+This part is the coding sequence of phytoene desaturase from <em>Erwinia uredovora</em>. Phytoene desaturase is part of carotenoid biosynthesis pathway and it takes responsible for convertion of phytoene to lycopene. A <em>Pea</em> transit peptide is fused with cds of phytoene desaturase in order to stablize and help phytoene desaturase folding in a correct formation in rice (<em>Oryza sativa</em>) cell. Codon optimized has been made for the expression in rice.
 <!-- Add more about the biology of this part here