Difference between revisions of "Part:BBa K1997014"

 
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<partinfo>BBa_K1997014 short</partinfo>
 
<partinfo>BBa_K1997014 short</partinfo>
  
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This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI
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This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 04:26, 17 October 2016


GFP10->Zif268->GFP11->RBS->GFP1-9

This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 208
    Illegal AgeI site found at 292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 380
    Illegal BsaI.rc site found at 112
    Illegal BsaI.rc site found at 443