Difference between revisions of "Part:BBa K1997024:Design"
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===Design Notes=== | ===Design Notes=== | ||
dCas9 was codon optimized for expression in E.coli | dCas9 was codon optimized for expression in E.coli | ||
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===Source=== | ===Source=== | ||
Latest revision as of 03:20, 17 October 2016
P+R->sHRP-N->dCas9->Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 415
Illegal NheI site found at 2038 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 657
Illegal BamHI site found at 4317 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
dCas9 was codon optimized for expression in E.coli
Source
This part is assembled from K1997000 and a dCas9 fragment