Difference between revisions of "Part:BBa K1949101:Design"
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===Design Notes=== | ===Design Notes=== | ||
sequence confirmed | sequence confirmed | ||
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| + | ===Materials and Methods=== | ||
====Construction==== | ====Construction==== | ||
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All the plasmids were prepared in XL1-Blue strain. | All the plasmids were prepared in XL1-Blue strain. | ||
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| + | ====Ⅰ.Adjustment of MazF Expression==== | ||
-Plasmids | -Plasmids | ||
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MazF : PBAD-<i>rbs-mazF-tt</i>-Pcon-<i>rbs-gfp</i> (pSB6A1) , Plac-<i>rbs</i> (pSB3K3) | MazF : PBAD-<i>rbs-mazF-tt</i>-Pcon-<i>rbs-gfp</i> (pSB6A1) , Plac-<i>rbs</i> (pSB3K3) | ||
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| + | ====Assay protocol==== | ||
===References=== | ===References=== | ||
Revision as of 02:38, 17 October 2016
PBAD-rbs-mazF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
sequence confirmed
Materials and Methods
Construction
-Strain
All the plasmids were prepared in XL1-Blue strain.
Ⅰ.Adjustment of MazF Expression
-Plasmids
GFP : Pcon-rbs-gfp (pSB6A1), Plac-rbs (pSB3K3)
MazF : PBAD-rbs-mazF-tt-Pcon-rbs-gfp (pSB6A1) , Plac-rbs (pSB3K3)