Difference between revisions of "Part:BBa K1949101:Design"
Line 9: Line 9:
 
sequence confirmed
 
sequence confirmed
  
 +
====Construction====
 +
-Strain
  
 +
All the plasmids were prepared in XL1-Blue strain.
 +
 +
-Plasmids
 +
GFP :  Pcon-<i>rbs</i>-<i>gfp</i> (pSB6A1), Plac-<i>rbs</i> (pSB3K3)<br>
 +
 +
MazF : PBAD-<i>rbs-mazF-tt</i>-Pcon-<i>rbs-gfp</i> (pSB6A1) , Plac-<i>rbs</i> (pSB3K3)
  
  
 
===References===
 
===References===

Revision as of 02:34, 17 October 2016


PBAD-rbs-mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

sequence confirmed

Construction

-Strain

All the plasmids were prepared in XL1-Blue strain.

-Plasmids GFP : Pcon-rbs-gfp (pSB6A1), Plac-rbs (pSB3K3)

MazF : PBAD-rbs-mazF-tt-Pcon-rbs-gfp (pSB6A1) , Plac-rbs (pSB3K3)


References