Difference between revisions of "Part:BBa K2136010"

Revision as of 23:58, 16 October 2016

5' cassete for Chlamydomonas transgenic expression Synthethic cassette for algae expression.

This composite part is the 5' part of a system to express proteins in Chlamydomonas reinhardtii. It includes a promoter, a resistance gene, a self cleaving peptide and a signal peptide. This combination, in conjunction with a in-frame coding sequence and a microalgae terminator enables the efficient translation and secretion to the growing media of the constructs of interest.

This part was characterized through the expression of a codon optimized version of mCherry reporter protein (https://parts.igem.org/Part:BBa_K2136016).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 264
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1539
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 1344
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2136010 short</partinfo>
+Synthethic cassette for algae expression.
+This composite part is the 5' part of a system to express proteins in ''Chlamydomonas reinhardtii''.
+It includes a promoter, a resistance gene, a self cleaving peptide and a signal peptide. This combination, in conjunction with a in-frame coding sequence and a microalgae terminator enables the efficient translation and secretion to the growing media of the constructs of interest.
+This part was characterized through the expression of a codon optimized version of mCherry reporter protein (https://parts.igem.org/Part:BBa_K2136016).
-Synthethic cassette for algae expression.
 <!-- Add more about the biology of this part here