Difference between revisions of "Part:BBa K1930004:Design"

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===References===
 
===References===
  
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[https://www.ncbi.nlm.nih.gov/gene/20467117 ''Gene ID'']
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<p>[https://www.ncbi.nlm.nih.gov/gene/20467117 ''Gene ID'']</p>
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[https://www.ncbi.nlm.nih.gov/pubmed/19822894 ''Quinolone resistance'']
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<p>[https://www.ncbi.nlm.nih.gov/pubmed/19822894 ''Quinolone resistance'']</p>

Revision as of 23:40, 16 October 2016


Ciprofloxacin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.


Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Gene ID

Quinolone resistance