Difference between revisions of "Part:BBa K1930004:Design"

 
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===Design Notes===
 
===Design Notes===
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To design a QnrS1 resistance cassette BioBrick BBa_K1930004 we designed a gBlock that contains the <em>Bacillus subtilis</em> promoter P<sub>AtpI</sub>, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original QnrS1 gene sequence from <em>E. coli</em>, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts P<sub>AtpI<sub>+RBS+QnrS1+2TER.
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To design a ciprofloxacin resistance cassette we designed a gBlock that contains the <em>Bacillus subtilis</em> promoter P<sub>AtpI</sub>, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original <em>qnrS1</em> gene sequence from <em>E. coli</em>, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts P<sub>AtpI</sub>+RBS+<em>qnrS1</em>+2TER.
  
  

Revision as of 23:37, 16 October 2016


Ciprofloxacin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.


Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References