Difference between revisions of "Part:BBa K1930004"
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<p>This part is a ciprofloxacin resistance cassette. It is containing a P<sub>AtpI</sub> constitutive promoter, ciprofloxacin resistance gene <em>qnrS1</em> and double terminator.</p>
 
<p>This part is a ciprofloxacin resistance cassette. It is containing a P<sub>AtpI</sub> constitutive promoter, ciprofloxacin resistance gene <em>qnrS1</em> and double terminator.</p>
  
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<p>The promoter P<sub>AtpI</sub> (BBa_K1930005) is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.</p>
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<p>The promoter P<sub>AtpI</sub> (<a href="https://parts.igem.org/Part:BBa_K1930005">BBa_K1930005</a>) is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.</p>
  
 
<p>Ciprofloxacin resistance gene <em>qnrS1</em> is found naturally in <em>E. coli</em>, and other Gram-negative strains. <em>qnr</em> genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.</p>
 
<p>Ciprofloxacin resistance gene <em>qnrS1</em> is found naturally in <em>E. coli</em>, and other Gram-negative strains. <em>qnr</em> genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.</p>

Revision as of 23:33, 16 October 2016


Ciprofloxacin resistance cassette

This part is a ciprofloxacin resistance cassette. It is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator.

The promoter PAtpI (<a href="https://parts.igem.org/Part:BBa_K1930005">BBa_K1930005</a>) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins. These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones.

Double terminator (BBa_B0015) is the most commonly used terminator. For more information see here: https://parts.igem.org/Part:BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]