Difference between revisions of "Part:BBa K1930005"

Revision as of 22:23, 16 October 2016

P_AtpI

The promoter P_AtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter P_AtpI in the pSB1C3 backbone to make it available to other iGEM teams.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1930005 short</partinfo>
-The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 min of germination. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.
+The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.
 The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter P<sub>AtpI</sub> in the pSB1C3 backbone to make it available to other iGEM teams.