Difference between revisions of "Part:BBa K1936001:Design"
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===Source=== | ===Source=== | ||
Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources. | Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources. | ||
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Tom5 yeast sequence:<html><br></html> | Tom5 yeast sequence:<html><br></html> | ||
http://www.ebi.ac.uk/ena/data/view/AY558369 | http://www.ebi.ac.uk/ena/data/view/AY558369 | ||
| − | |||
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LOV2 sequence:<html><br></html> | LOV2 sequence:<html><br></html> | ||
from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | ||
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===References=== | ===References=== | ||
<html><font size="-1">[1]</font><font size="-2"> Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.</font></html> | <html><font size="-1">[1]</font><font size="-2"> Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.</font></html> | ||
Revision as of 21:11, 16 October 2016
Tom5-eGFP-LOV2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 33
Illegal BamHI site found at 712
Illegal XhoI site found at 515 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1226
Design Notes
ALERT !!!Under construction !!
Source
Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.
Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369
LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]
References
[1] Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.