Difference between revisions of "Part:BBa K1936001:Design"
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LOV2 sequence:<html><br></html> | LOV2 sequence:<html><br></html> | ||
− | from pKM291 | + | from pKM291 containing LOVpep[T406A,T407A] double mutant [1] |
===References=== | ===References=== |
Revision as of 21:09, 16 October 2016
Tom5-eGFP-LOV2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 33
Illegal BamHI site found at 712
Illegal XhoI site found at 515 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1226
Design Notes
ALERT !!!Under construction !!
Source
Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.
Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369
eGFP sequence:
LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]